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HomeRedesPlataforma de fenotipos y ensayos de campo

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Ensayos de campo y plataformas de fenotipado

El análisis genético (mapeo de QTL) y el análisis ecofisiológico se realizaron a partir de grandes bases de datos recogidas en ensayos de campo y de plataforma. Los experimentos de campo tenían como objetivo determinar la respuesta del rendimiento de los paneles SolACE a las variaciones en la disponibilidad de recursos. Las plataformas de fenotipado y los experimentos en condiciones controladas tenían como objetivo recoger datos sobre la arquitectura de las raíces y la respuesta del microbioma a las imitaciones de recursos combinados.

Se probó una amplia gama de genotipos de trigo en cuatro ensayos de campo en condiciones de control y de estrés combinado. Se probaron 250 genotipos de trigo pan en ensayos de campo en Levroux (Francia) y Gréoux-les-Bains (Francia). Otros 250 genotipos de trigo duro se analizaron en ensayos de campo en Foggia (Italia) y Mauguio (Francia). Además, los paneles de gran diversidad fueron fenotipados a nivel de la raíz en ensayos de la plataforma de fenotipado en Lovaina (Bélgica) y Dijon (Francia).

En una segunda fase, se seleccionaron 80 genotipos (40 de trigo pan y 40 de trigo duro) para analizarlos con mayor detalle en ensayos de campo semicontrolados en Gréoux-les-Bains y Clermont (Francia) y Copenhague (Dinamarca), así como en una plataforma de fenotipado en Montpellier (Francia).

Además de los ensayos con trigo duro y pan, también se probaron 24 variedades de patata en una plataforma de invernadero en Dundee, Escocia.

La información genética procedente de estos ensayos a gran escala y específicos se combinó en un marco de modelización que combina modelos estructurales funcionales de plantas (FSPM) y modelos de cultivos con el fin de mejorar nuestra comprensión del rendimiento de los cultivos bajo limitaciones.

Noticias sobre las pruebas de fenotipo

Selection of subsets of genotypes of bread wheat and durum wheat

Eighty genotypes of wheat (40 out of 250 bread wheat and 40 out of 250 durum wheat) were selected to be analysed in greater detail in the upcoming phenotyping platform trials and semi-controlled field trials. The genotype selection was an important step in order to ensure a large amplitude of response variation, within the subsets of genotypes, under combined limitation of water and nitrogen.

SolACE bread wheat field trial in Gréoux-les-Bains conducted by Arvalis. Photo: Marco Cosme, June 2018.

Figure 1: Contrasted response of the bread wheat SolACE panel (250 genotypes). Selected genotypes are marked with crosses (meaning of colours: see legend). Pierre Martre. September 2018.


One of the goals of the SolACE project is to better understand crop and microbiome responses to combined water and nutrient limitations. The genotype selection therefore aimed to ensure a high amplitude of genotype responses in the phenotyping platform and field trials. In order to achieve this, data were collected from various sources:

  • Four SolACE field trials located in France and Italy,
  • Experiments carried out under controlled conditions by SolACE project partners (e.g., an experiment carried out by the Université catholique de Louvain (UCL) about the root angle of young seedlings).
  • Data coming from prior field and platform trials (e.g., ArchiRac project, ARVALIS data).  

The data were organized into huge data files, with about 250 lines coming from the bread wheat diversity panel and about 250 genotypes from the durum wheat diversity panel. It was decided to separate the analyses between bread and durum wheat to enable more focused discussions and analyses.

The 40 genotypes of bread wheat were selected based on the ‘LEPSE method’: each genotype was assigned a score according to its response to different environments. The sum of these scores was used to compare the genotypes as it can be considered as a good indicator of stress response across the different environments. The highest values were selected as “tolerant” and the lowest as “sensitive” (see Figure 1). Within those genotypes, care was taken to also keep genotypes with contrasting root traits as well as hybrids and their parents. This method was selected because it kept the largest diversity of response among the 40 selected genotypes.

To ensure a wide variety of responses in the selection of durum wheat, each of the three section from the whole panel was analyzed individually. For each section, the genotypes with the highest and lowest score (as explained above in bread wheat) were selected to form a group of 40 contrasting genotypes coming from the different sections.

We will now conduct more detailed platform and field trials to deeper understand how the selected genotypes react to a combined stress in water and nitrogen and carry out further (eco-)physiological characterization.

Further information

Contact

Clothilde Collet, Université Catholique de Louvain

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