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HomeRedesPlataforma de fenótipo e ensaios de campo

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Ensaios de campo e plataformas de fenotipagem

A análise genética (mapeamento QTL) e a análise ecofisiológica foram realizadas com base em grandes bases de dados recolhidas de ensaios de campo e de plataforma. As experiências de campo visavam determinar a resposta de rendimento dos painéis SolACE a variações na disponibilidade de recursos. Plataformas de fenotipagem e experiências em condições controladas destinadas a recolher dados sobre a arquitectura de raiz e a resposta microbiológica a imitações combinadas de recursos.

Uma vasta gama de genótipos de trigo foi testada em quatro ensaios de campo sob controlo e condições de stress combinadas. 250 genótipos de trigo panificável foram testados em ensaios de campo em Levroux, França e Gréoux-les-Bains, França. Outros 250 genótipos do trigo duro foram testados em ensaios de campo em Foggia, Itália e Mauguio, França. Além disso, os grandes painéis de diversidade foram fenotípicos a nível da raiz em ensaios de plataforma de fenotipagem em Lovaina, Bélgica e Dijon, França.

Numa segunda fase, 80 genótipos seleccionados (40 de trigo panificável e 40 de trigo duro) foram seleccionados para serem analisados em maior detalhe em ensaios de campo semi-controlados em Gréoux-les-Bains e Clermont, França e Copenhaga, Dinamarca, bem como numa plataforma de fenotipagem em Montpellier, França.

Para além dos ensaios de pão e trigo duro, 24 variedades de batata estavam também a ser testadas numa plataforma de estufa de batata em Dundee, Scottland.

A informação genética destes ensaios em grande escala e orientados foram combinados num quadro de modelização que associa modelos de plantas estruturais funcionais (FSPM) e modelos de culturas, a fim de melhorar a nossa compreensão do desempenho das culturas sob limitações.

Notícias sobre os ensaios de fenótipo

Selection of subsets of genotypes of bread wheat and durum wheat

Eighty genotypes of wheat (40 out of 250 bread wheat and 40 out of 250 durum wheat) were selected to be analysed in greater detail in the upcoming phenotyping platform trials and semi-controlled field trials. The genotype selection was an important step in order to ensure a large amplitude of response variation, within the subsets of genotypes, under combined limitation of water and nitrogen.

SolACE bread wheat field trial in Gréoux-les-Bains conducted by Arvalis. Photo: Marco Cosme, June 2018.

Figure 1: Contrasted response of the bread wheat SolACE panel (250 genotypes). Selected genotypes are marked with crosses (meaning of colours: see legend). Pierre Martre. September 2018.


One of the goals of the SolACE project is to better understand crop and microbiome responses to combined water and nutrient limitations. The genotype selection therefore aimed to ensure a high amplitude of genotype responses in the phenotyping platform and field trials. In order to achieve this, data were collected from various sources:

  • Four SolACE field trials located in France and Italy,
  • Experiments carried out under controlled conditions by SolACE project partners (e.g., an experiment carried out by the Université catholique de Louvain (UCL) about the root angle of young seedlings).
  • Data coming from prior field and platform trials (e.g., ArchiRac project, ARVALIS data).  

The data were organized into huge data files, with about 250 lines coming from the bread wheat diversity panel and about 250 genotypes from the durum wheat diversity panel. It was decided to separate the analyses between bread and durum wheat to enable more focused discussions and analyses.

The 40 genotypes of bread wheat were selected based on the ‘LEPSE method’: each genotype was assigned a score according to its response to different environments. The sum of these scores was used to compare the genotypes as it can be considered as a good indicator of stress response across the different environments. The highest values were selected as “tolerant” and the lowest as “sensitive” (see Figure 1). Within those genotypes, care was taken to also keep genotypes with contrasting root traits as well as hybrids and their parents. This method was selected because it kept the largest diversity of response among the 40 selected genotypes.

To ensure a wide variety of responses in the selection of durum wheat, each of the three section from the whole panel was analyzed individually. For each section, the genotypes with the highest and lowest score (as explained above in bread wheat) were selected to form a group of 40 contrasting genotypes coming from the different sections.

We will now conduct more detailed platform and field trials to deeper understand how the selected genotypes react to a combined stress in water and nitrogen and carry out further (eco-)physiological characterization.

Further information

Contact

Clothilde Collet, Université Catholique de Louvain

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