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Ensaios de campo e plataformas de fenotipagem

A análise genética (mapeamento QTL) e a análise ecofisiológica foram realizadas com base em grandes bases de dados recolhidas de ensaios de campo e de plataforma. As experiências de campo visavam determinar a resposta de rendimento dos painéis SolACE a variações na disponibilidade de recursos. Plataformas de fenotipagem e experiências em condições controladas destinadas a recolher dados sobre a arquitectura de raiz e a resposta microbiológica a imitações combinadas de recursos.

Uma vasta gama de genótipos de trigo foi testada em quatro ensaios de campo sob controlo e condições de stress combinadas. 250 genótipos de trigo panificável foram testados em ensaios de campo em Levroux, França e Gréoux-les-Bains, França. Outros 250 genótipos do trigo duro foram testados em ensaios de campo em Foggia, Itália e Mauguio, França. Além disso, os grandes painéis de diversidade foram fenotípicos a nível da raiz em ensaios de plataforma de fenotipagem em Lovaina, Bélgica e Dijon, França.

Numa segunda fase, 80 genótipos seleccionados (40 de trigo panificável e 40 de trigo duro) foram seleccionados para serem analisados em maior detalhe em ensaios de campo semi-controlados em Gréoux-les-Bains e Clermont, França e Copenhaga, Dinamarca, bem como numa plataforma de fenotipagem em Montpellier, França.

Para além dos ensaios de pão e trigo duro, 24 variedades de batata estavam também a ser testadas numa plataforma de estufa de batata em Dundee, Scottland.

A informação genética destes ensaios em grande escala e orientados foram combinados num quadro de modelização que associa modelos de plantas estruturais funcionais (FSPM) e modelos de culturas, a fim de melhorar a nossa compreensão do desempenho das culturas sob limitações.

Notícias sobre os ensaios de fenótipo

Understanding crop and microbiome responses to combined water and nutrient limitations

Part of the SolACE project analyses crop and microbiome responses to combined water and nutrient limitations. Genetic information from large-scale field and platform trials and physiological information from targeted trials are combined in a modelling framework, which couples Functional Structural Plant Models (FSPM) and crop models in order to improve our understanding of crop performance under limitations.

Map of ongoing SolACE field and platform experiments.

Diversity panels (approximately 250 genotypes) of bread and durum wheat and a collection of 24 potato varieties have been defined and assembled. The selection was made by the French National Institute of Agronomic Research (INRA), the National Research Council (CREA) in Italy, ARVALIS, the UK-Based James Hutton Institute and SOLYNTA and was based on a priori genetic and phenotypic information.

Two field trials were installed for each of the wheat panels during autumn 2017. Bread wheat trials were sown in Levroux (SYNGENTA, France) and Gréoux-les-Bains (ARVALIS, France), and durum wheat trials were sown in Foggia (CREA, Italy) and Mauguio (INRA, France). At all places, each genotype will be exposed to combined water and nitrogen limitations and compared to a control scenario. Field partners are currently working with modellers to establish the final list of variables that will be measured.

The 24 potato varieties have gone through a first greenhouse assay to confirm the interest of the collection. The next trials are in preparation. They will focus on root system architecture, yield and canopy development, and will come up with a novel diagnostic transcriptome test.

We will soon begin the high-throughput root phenotypic platform trials that will be performed at Université catholique de Louvain (Belgium) and at INRA-Dijon (France).
By the end of the summer, the field and platform data from wheat experiments will be analysed together and discussed broadly with partners to determine a short collection of 40 genotypes that will be analysed in more detail within intensified field and platform trials.

More information about work package 2.

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